一、教育、工作经历：

2025年5月-至今 东莞理工学院，生命健康技术学院，学科领军，研究员

2013年7月-2025年4月 广东省科学院生态环境与土壤研究所，助理研究员、副研究员、研究员

2010年9月-2013年6月 中国科学院广州生物医药与健康研究院，博士

2007年9月-2010年6月 暨南大学，生命科学技术学院，硕士

2002年9月-2006年6月 遵义医科大学，生物工程学院，学士

二、研究领域：

1. 食品及环境安全检测新理论和新方法

2. 疾病生物标志物分子诊断新技术

3. DNA纳米技术智能生物传感器

三、主要业绩：

长期从事食品及环境安全方面的研究工作，涉及污染物检测新方法构建、配套检测装备研发、智能化生物传感器研制等，实现了对重金属、新污染物、致病菌、农兽药残留、真菌毒素、生物标志物等的快速检测与筛查。以第一/通讯作者在Angewandte Chemie、Chemical Science、Analytical Chemistry、Food Chemistry、Journal of Agricultural and Food Chemistry等期刊发表SCI论文60多篇（其中自然指数论文20多篇）；授权发明专利30多项，多项技术实现成果转化。先后主持国家、省部级项目10余项；入选了广东省杰青、广东省特支计划青年拔尖人才、3551光谷人才计划-创新（长期）人才、珠江人才计划创新团队核心成员、珠江科技新星、广东省现代农业产业技术体系岗位专家等。

四、近5年代表性论文

1. Jiafeng Pan, Ying Zeng, Yuyan Wang, Xiaoya Ren, Fei Yang*, Junhua Chen*. Logic circuits for intelligent microcystin monitoring based on aptamer recognition and toehold-mediated hairpin DNA self-assembly. Analytical Chemistry 2025, 97, 2556-2565.

2. Junhua Chen*, Xu Wang, Junhui Guo, Yiwen Lv, Manjia Chen, Hui Tong, Chengshuai Liu*. Heavy metal-induced assembly of DNA network biosensor from double-loop Hairpin probes for ultrasensitive detection of UO₂²⁺ in water and soil samples. Analytical Chemistry 2024, 96, 3153-3159.

3. Jiafeng Pan, Ying He, Zhi Liu, Junhua Chen*. Tetrahedron-based constitutional dynamic network for COVID-19 or other coronaviruses diagnostics and its logic gate applications. Analytical Chemistry 2022, 94, 714-722.

4. Jiafeng Pan, Fang Deng, Zhi Liu, Gu Shi, Junhua Chen*. Toehold-mediated cascade catalytic assembly for mycotoxin detection and its logic applications. Analytical Chemistry 2022, 94, 3693-3700.

5. Junhua Chen*, Gu Shi, Chong Yan. Visual test paper for on-site polychlorinated biphenyls detection and its logic gate applications. Analytical Chemistry 2021, 93, 15438-15444.

6. Shi Gu, Chong Yan, Junhua Chen*. Scalable logic circuits with multiple outputs and automatic reset function based on DNAzyme-mediated branch migration. Analytical Chemistry 2021, 93, 3273-3279.

7. Junhua Chen*, Jiafeng Pan, Chengshuai Liu. Versatile sensing platform for Cd²⁺ detection in rice samples and its applications in logic gate computation. Analytical Chemistry 2020, 92, 6173-6180.

8. Fang Deng, Jiafeng Pan, Zhi Liu, Lingwen Zeng, Junhua Chen*. Programmable DNA biocomputing circuits for rapid and intelligent screening of SARS-CoV-2 variants. Biosensors and Bioelectronics 2023, 223, 115025.

9. Gu Shi, Chong Yan, Junhua Chen*. Fluorescent aptasensor for the ultrasensitive detection of antibiotic residue in food samples based on dumbbell DNA-mediated signal amplification. Biosensors and Bioelectronics 2023, 228, 115188.

10. Jin Chen, Jiafeng Pan, Meijuan Duan, Wenbang Tang, Nenghui Ye, Junhua Chen*, Zhi Liu*, Dingyang Yuan*. In situ images of Cd²⁺ in rice reveal Cd²⁺ protective mechanism using DNAzyme fluorescent probe. Journal of Hazardous Materials 2025, 483, 136650.

11. Chong Yan, Gu Shi, Junhua Chen*. Fluorescent detection of two pesticides based on CRISPR-Cas12a and its application for the construction of four molecular logic gates. Journal of Agricultural and Food Chemistry 2022, 70, 12700-12707.

12. Gu Shi, Chong Yan, Junhua Chen*. Ultrasensitive aptasensor for microcystin-LR detection in food samples based on target-activated assembly of Y-shaped hairpin probes. Journal of Agricultural and Food Chemistry 2022, 70, 16446-16452.

13. Jiafeng Pan, Zhi Liu, Junhua Chen*. An amplifying DNA circuit coupled with Mg²⁺- dependent DNAzyme for bisphenol A detection in milk samples. Food Chemistry 2021, 346, 128975.

14. Jiafeng Pan, Ying He, Zhi Liu*, Junhua Chen*. Dual recognition elements-controlled logic DNA circuit for COVID-19 detection based on exonuclease III and DNAzyme. Chemical Communications 2021, 57, 1125-1128.

15. Junhua Chen, Manjia Chen, Hui Tong, Fei Wu, Yizhang Liu, Shuaicheng Liu*. Fluorescence biosensor for ultrasensitive detection of the available lead based on target biorecognition-induced DNA cyclic assembly. Science of The Total Environment 2023, 905, 167253.

16. Jiafeng Pan, Fang Deng, Junhua Chen*. A fluorescent biosensor for Cd²⁺ detection in water samples based on Cd²⁺-fueled wheel DNAzyme walker and its logic gate applications. Science of The Total Environment 2023, 864, 161046.

17. Junhua Chen*, Gu Shi, Chong Yan. Portable biosensor for on-site detection of kanamycin in water samples based on CRISPR-Cas12a and an off-the-shelf glucometer. Science of The Total Environment 2023, 872, 162279.

18. Fang Deng, Jiafeng Pan, Manjia Chen, Zhi Liu*, Junhua Chen*, Chengshuai Liu. Integrating CRISPR-Cas12a with catalytic hairpin assembly as a logic gate biosensing platform for the detection of polychlorinated biphenyls in water samples. Science of The Total Environment 2023, 881, 163465.

19. Fang Deng, Jiafeng Pan, Zhi Liu*, Junhua Chen*. Cascaded molecular logic gates using antibiotics as inputs based on exonuclease III and DNAzyme. Talanta 2023, 252, 123832.

20. Jiafeng Pan, Fang Deng, Zhi Liu, Lingwen Zeng, Junhua Chen*. Construction of molecular logic gates using heavy metal ions as inputs based on catalytic hairpin assembly and CRISPR-Cas12a. Talanta 2023, 255, 124210.

五、代表性授权发明专利：

1. 陈俊华，潘家峰，邓芳。分子逻辑门智能化检测真菌毒素新方法ZL2022101205662

2. 陈俊华，刘承帅，袁宇志，陈曼佳，童辉。一种检测抗生素的生物传感器ZL2024106671234

3. 陈俊华，刘承帅，潘家峰，邓芳。一种基于核酸四面体的冠状病毒定量检测方法及其应用ZL2021113650351

4. 陈俊华，李琼，周丹华，潘家峰。一种免标记荧光检测基因的方法及试剂盒ZL2019100576061

5. 陈俊华，周丹华，潘家峰，王琦。一种基于核酸适体的荧光检测试剂盒ZL2017109482338

6. 陈俊华，周顺桂，温俊林。一种汞离子的超灵敏检测方法及检测试剂盒ZL2014100050848

六、主持科研项目：

1. 广东省杰出青年基金，土壤污染物快速检测的分子探针设计与试剂盒研制，2016-2020。

2. 广东省特支计划科技创新青年拔尖人才项目，2016-2019。

3. 国家自然科学基金面上项目，基于抗生素智能化识别与传感的多通道分子逻辑门构建，2024-2027。

4. 广东省现代农业共性关键技术创新团队岗位专家项目，污染物检测新技术，2025-2027。

5. 国家重点研发计划课题，珠三角经济区基本农田质量及生态监测技术研究与示范，2015-2019。

6. 3551光谷人才计划-创新（长期）人才项目，重金属检测技术研究与应用，2020-2022。